Book of Abstracts: Albany 2007

category image Albany 2007
Conversation 15
June 19-23 2007

Multiple-Helix Bundles and Their Applications in DNA Nanotechnology

One of the key goals in structural DNA nanotechnology is to build highly-ordered structures self-assembled from individual DNA motifs in 1D, 2D, and finally 3D. The most successful examples of self-assembly are programmable 2D periodic lattices that are held together by double cohesion. We have reported several DNA motifs that are inherently 3D because they contain three independent vectors that span 3-dimensional space (1). For example, the designed cyclic DNA motif (6HB) that consists of six DNA double helices, in which two adjacent double helices are connected to each other at two crossover points.

We designed two new motifs based on the 6HB bundle motif: eight-helix bundles (8HB) and nine-helix bundles (9HB). Similar to 6HB, two crossover points connect two adjacent double helices in 9HB. The 8HB motif was originally designed to produce 2D periodic arrays, and the 9HB motif was designed to yield 3D self-assembled arrays. Both motifs have been successfully used to produce 2D arrays via double cohesion. 3D crystals have resulted from the self-assembly of 9HB, but they do not display the unit cells expected from the design.

This research supported by NIGMS, NSF, ARO and the W.M. Keck Foundation.

References and Footnotes
  1. Constantinou, P., Wang, T., Kopatsch, J., Israel, L. B., Mao, C., Ding, B., Sha, R., Zhang, X., Yang, X., and Seeman, N. C. Org Biomol Chem 4, 3414-3419 (2006).

Tong Wang
Nadrian C. Seeman*

Department of Chemistry
New York University
New York NY 10003

*Phone: 212-998-8395
Fax: 212-260-7905
Email: ned.seeman@nyu.edu