Book of Abstracts: Albany 2009

category image Albany 2009
Conversation 16
June 16-20 2009
© Adenine Press (2008)

Metabolome Analysis and Systems Biology

Institute for Advanced Biosciences of Keio University has recently developed a novel technology for high-throughput metabolome analysis. The technology is based on capillary electrophoresis time-of-flight mass spectrometry (CE/TOFMS) and it can simultaneously quantify a large amount of cellular metabolites ranged from 70 to 1,000 molecular weights [1]. Metabolome analysis is applicable to various fields of biotechnology in the post-genomic era, such as medical diagnosis (blood, urine, tissue), food production (farm products, fermentation), and systems biology of model organisms (E.coli and other bacteria).

The metabolome technology has made ?multi-omics? analysis possible. We systematically obtained multi-omics data sets for Escherichia coli BW25113 and its single gene deletion mutants. Our data covers the metabolome (CE-TOFMS), proteome (western blot, shotgun proteomics, and 2D-DIGE), fluxome (GC-MS and NMR) and transcriptome (real time RT-PCR and DNA?microarray) [4].

In the area of medical diagnosis, we recently discovered a biomarker of acetaminophen-induced hepatotoxicity, ophthalmate being a sensitive?biomarker of glutathione depletion [2]. In addition, Metabolome data have?been used to confirm simulation results of red blood cell metabolism [3].

References and Footnotes
  1. J. Proteome Res. 2: 488-494 (2003).
  2. J. Biol. Chem. 281: 16768-16776 (2006).
  3. J. Biol. Chem. 282; 10731-107341(2007).
  4. Science 316, 593-597 (2007).

Masaru Tomita

Institute for Advanced Biosciences
Keio University
Fujisawa, 252-8520, Japan

email: mt@sfc.keio.ac.jp