Book of Abstracts: Albany 2003

category image Albany 2003
Conversation 13
Abstract Book
June 17-21 2003

Investigations of cis-Pt and its Analog DACH-Pt Interaction with DNA and Synthetic Oligonucleotides by Means of VCD, IR and CD Spectroscopy

We studied the interaction of cis-Pt and its more effective analog 1,2-diaminocyclo-hexane-platinum (II) (DACH-Pt) with natural calf thymus DNA as well as the interaction of DACH-Pt with the synthetic octamer duplex d(CCTGGTCC)·d(GGACCAGG) using Vibrational Circular Dichroism (VCD), IR and UV/CD. We measured these spectra at different temperatures between 25 to 70 °C for calf thymus DNA and between 4 to 55 °C for the octamer duplex. We obtained different spectral changes for DNA interacting with cis-Pt than with DACH-Pt. DACH-Pt showed strong destabilization of the octamer duplex. We were able to conclude that DACH-Pt possesses a higher ability for destabilizing the octamer duplex than ordinary cis-Pt. The results are compared for octamer duplex-DACH-Pt complex and octamer duplex-cis-Pt studied previously on our laboratory (1).

Valery Andrushchenko1
Alexander Polyanichko1
Berndt Kalisch2
Hal Wieser1

1Department of Chemistry
Faculty of Science
University of Calgary
2500 University Dr. NW
Calgary, AB
T2N 1N4, Canada
2Department of Biochemistry and Molecular Biology
Faculty of Medicine
Heritage Medical Research Bldg.
3330 Hospital Drive N.W.
Calgary, AB
T2N 1N4, Canada

References and Footnotes
  1. D. Tsankov, Berndt Kalisch, Hans van de Sande, and H. Wieser, J. Phys. Chem. B., accepted for publication.