Albany 2015:Book of Abstracts

Albany 2015
Conversation 19
June 9-13 2015
©Adenine Press (2012)

Integrating Multi-Scale Data on Homologous Recombination into a New Recognition Mechanism based on Simulations of the RecA-ssDNA/dsDNA Structure

RecA protein is the prototypical recombinase. Members of the recombinase family can accurately repair double strand breaks in DNA. They also provide crucial links between pairs of sister chromatids in eukaryotic meiosis. A very broad outline of how these proteins align homologous sequences and promote DNA strand exchange has long been known, as are the crystal structures of the RecA-DNA pre- and postsynaptic complex; however, little is known about the homology searching conformations and the details of how DNA in bacterial genomes is rapidly searched until homologous alignment is achieved. By integrating a physical model of recognition to new modeling work based on docking exploration and molecular dynamics simulation, we present a detailed structure/function model of homology recognition that reconciles extremely quick searching with the efficient and stringent formation of stable strand exchange products and which is consistent with a vast body of previously unexplained experimental results.

Darren Yang1
Benjamin Boyer2
Chantal Prévost2
Claudia Danilowicz3
Mara Prentiss1, 3

1School of Engineering and Applied Sciences
Harvard University
Cambridge, MA 02138, USA
2Laboratoire de Biochimie Theorique
IBPC, Paris, France
3Department of Physics
Harvard University
Cambridge, MA 02138, USA

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