Book of Abstracts: Albany 2011

category image Albany 2011
Conversation 17
June 14-18 2011
©Adenine Press (2010)

Crystalline Two-Dimensional DNA-Origami Arrays

Nanotechnology aims to organize matter with the highest possible accuracy and control. Such control will lead to nanoelectronics, nanorobotics, programmable chemical synthesis, scaffolded crystals, and nanoscale systems responsive to their environments. Structural DNA nanotechnology (1) is one of the most powerful routes to this goal. It combines robust branched DNA species with the control of affinity and structure (2) inherent in the programmability of sticky ends. The successes of structural DNA nanotechnology include the formation of objects (3), 2D crystals (4), 3D crystals (5), nanomechanical devices (6), and various combinations of these species (7). DNA origami (8) is arguably the most effective way of producing a large addressable area on a 2D DNA surface. This method entails the combination of a long single strand (typically the single-stranded form of the filamentous bacteriophage M13, 7249 nucleotides) with about 250 staple strands to define the shape and patterning of the structure. With a pixelation estimated at about 6 nm (8), it is possible to build patterns with about 100 addressable points within a definable shape in an area of about 10000 nm2. Many investigators have sought unsuccessfully to increase the useful size of 2D origami units by forming crystals of individual origami tiles (9). Herein, we report a double-layer DNA-origami tile with two orthogonal domains underwent self-assembly into well-ordered two-dimensional DNA arrays with edge dimensions of 2–3 μm (see schematic representation and AFM image). This size is likely to be large enough to connect bottom-up methods of patterning with top-down approaches.

Two-dimensional crystals from DNA origami tiles


This research has been supported by the following grants to NCS: GM-29544 from the National Institute of General Medical Sciences, CTS-0608889 and CCF-0726378 from the National Science Foundation, 48681-EL and W911NF-07-1-0439 from the Army Research Office, N000140910181 and N000140911118 from the Office of Naval Research and a grant from the W.M. Keck Foundation.


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Wenyan Liu
Hong Zhong
Risheng Wang
and Nadrian C. Seeman

Department of Chemistry
New York University
New York, NY 10003, USA

Ph: (212) 998-8395
Fx: (212) 260-7905