Albany 2013: Book of Abstracts

category image Albany 2013
Conversation 18
June 11-15 2013
©Adenine Press (2012)

Circular dichroism (CD) studies of the interaction between G-quadruplexes/ I-motif with saffron secondary active metabolites

Telomeric DNA contains some unique secondary structures, such as G-quadruplex and I-motif. These structures may be stabilized or changed by binding to specific proteins or small molecules. In continue with our previous studies on the interaction between crocin and crocetin, as the natural C20 carotenoids, and picrocrocin and safranal, as the natural monoterpene aldehydes, obtained from saffron and DNA (Bathaie et al. 2007; Hoshyar et al. 2008), herein, we report the in vitro effect of these saffron metabolites on the mentioned structures (Hoshyar et al. 2012). Circular dichroism (CD) data indicate crocetin has higher affinity for these structures. Safranal and crocin induce little change in the I-motif and G-quadruplex, respectively. The molecular docking confirms the experimental data and indicates the minor groove binding of ligands with G-quadruplex. Effect of these ligands on the stability of these structures are studying using some other techniques and determination of thermodynamic parameters.


    S.Z. Bathaie, A. Bolhasani, R. Hoshyar, B. Ranjbar, F. Sabouni and A.A. Moosavi-Movahedi, (2007). DNA and Cell Biology, 26, 533-540.

    R. Hoshyar, S.Z. Bathaie, M. Ashrafi (2008). DNA and Cell Biology, 27 (12), 1-9.

    R. Hoshyar, S.Z. Bathaie, A. Kyani and M.F. Mousavi (2012). Nucleoside, Nucleotide, Nucleic Acids, 31 (11), 801-812.

S.Zahra Bathaie
Reyhane Hoshyar
Nasim Shahhamzeie

Department of Clinical Biochemistry
Faculty of Medical Sciences
Tarbiat Modares University
P.O.Box: 14115-111
Tehran, Iran. zbatha2000@yahoo.com