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Albany 2019: 20th Conversation - Abstracts

category image Albany 2019
Conversation 20
June 11-15 2019
Adenine Press (2019)

The Distribution of 3, 5, 3’-Triiodothyronine between the Transport Systems of Blood and Nuclei of the Tissues

It is known that hydrophobic hormones can be transporting in blood by two mechanisms: in serum proteins complex and in internal volume of erythrocytes (Dolomatov et. al, 1999, Eliseeva et.al., 2009, Gimatdinova et al., 2011, Garipova et.al., 2010, 2013, 2015). It is interesting to answer the question of how the intracellular hormone concentration is related to it is concentration in each of hormone transporting systems. There distribution of 3,5,3’-triiodothyronine (T3) between the transport systems of blood and nuclei of leucocytes, lung and liver tissues of rat were examined. The 3,5,3'-triiodothyronineconcentrations in blood plasma, hemolysate and nucleoplasm prepared from leucocytes, lung and liver of 10 laboratory non-linear male rats were determined by enzyme immunoassay. The 3,5,3’-triiodothyronine concentration in blood plasma was averaged 6, 33±0, 34 pmol/l. The T3 concentration in erythrocyte internal volume (hemolysate) was reliably less- 2, 94 pmol/l (Т=8, 34, р=0,001).This indicates relative depletion of erythrocyte triiodothyronine depot. T3 concentrations in tissue nucleus were dramatically more, than in blood hormone transporting systems. The triiodothyronine concentration in leukocytenucleiwas2, 57times more than in blood plasma (16, 24±0,98pmol/l). Inlung nuclei T3 concentration was 12, 23±0, 87 pmol/l and in liver nucleoplasm 16, 68±1, 03 pmol/l. Perhaps, triiodothyronine concentration is associated with biosynthetic activity of the tissue: correlation coefficient between T3 and RNA concentrations in nucleoplasm was 0, 57 (р=0,003).

The distribution of triiodothyronine between blood plasma, internal volume of erythrocytes and blood leukocyte nuclei of healthy donors were studied. It’s shown, that in blood plasma T3 concentration is 4,14 ±0,6 pmol/l, that is 17,6 % of total hormone content in blood sample. The triiodothyronine concentration in erythrocytes was 6, 05±0, 4 pmol/l that is25, 8 % of total hormone content. It is noteworthy, that the T3 concentration in leucocyte nucleoplasm averaged 13, 3 ± 0,6pmol/l, that is more than 3 times higher than the hormone concentration in blood plasma. Thus, both in the nuclei of laboratory rats tissues and healthy donors T3 concentration in nuclei significantly exceeds the T3 concentration in both blood hormone transporting systems, which indicates the mechanisms of active transport and accumulation of 3,5,3'-triiodothyroninein the tissue nuclei.

References

    Dolomatov S.I., Pishak V. P., Slipenuk T. S., Meshishen I. F., Okopnaya T.V. (1999). Erythrocyte capacity to depose thyroid hormones: regulatory function of physicochemical factors in vitro. Voprosy Med. Chimii, â„– 6, 572-577.

    Garipova M.I., Usmanova R.R. Isolation and partial characterization of a general hormone transporting blood protein complex./Journal of Biomolecular Structure and Dynamics, 2013-Vol. 31, No. S1, p.118.

    Garipova M.I., Dazko O.I. Two differenthormonetransporting systems inhuman blood:features of peptide hormone transport in human blood /Journal of Biomolecular Structure and Dynamics.- 2015.-Vol. 33.- No. S1, p.162.

    Eliseeva O. S., Kireeva N. A., Pershina A. S., Garipova M.I. (2009). Investigation of the insulin interaction with the erythrocytes surface. Vestnik OGU, N 6, 476-478.

    Garipova M.I., Morugova T.V., Kireeva N.A., Ibragimov R.I., Baranova M.V. (2010). Affinity preparation and insulin-binding human blood serum protein diversity investigation. Voprosy Med. Chimii, â„– 8, 40-44.

    Gimatdinova E.V., Hayrullina R.M., Garipova M.I., Sotnikova U.M., Veselov S.U. (2011) Diagnostic and prognostic opportunities of procalcitonin and c-reactive protein in different infectious and inflamatory prosess with children. Fundamental Research, â„– 10-2, 280-282.

Margarita I. Garipova
Alfira I. Shigapova
Rashit G. Farkhutdinov
Vadim V. Fedyaev
Julia M. Sotnikova
Alfira B. Yakupova

Department of Biology
Bashkir State University
450076, Ufa
Z. Validy, 32, Russia

Ph: (347)2299671
Fax: (347)273-67-78
Email: margaritag@list.ru