Martin Gruebele, University of Illinois, will describe how the quinary interactions, sticking and crowding affect protein folding and protein-protein interactions in the cytoplasm. Trends from conflicting pressures of sticking and crowding will be unraveled by comparing in-cell with in vitro experiments, and new techniques to examine protein-protein interaction dynamics and thermodynamics inside cells reveal important differences to in vitro assays. Systems range from metabolic enzymes, to loose protein clusters, to the spliceosome.