Book of Abstracts: Albany 2007

category image Albany 2007
Conversation 15
June 19-23 2007

Direct excitation luminescence spectroscopy of lanthanide ions bound to DNA and RNA

The luminescence spectra of lanthanide ions bound to DNA or RNA are highly dependent on nucleic acid sequence and structure. For example, a simple DNA hairpin with the naturally occurring GCA loop is a binding site for europium ion. Direct excitation luminescence spectroscopy shows that europium ion luminescence intensity and lifetime increase upon addition of the DNA hairpin. These excitation spectra and time-resolved studies are consistent with loss of europium water ligands in the europium-DNA complex. The hairpin binding site is exquisitely sensitive to single nucleotide changes in both the loop and stem. The luminescence signature of lanthanides bound to various DNA and RNA oligonucleotides containing common structural motifs will be presented. The characterization of metal ion binding sites is important to the design of luminescent nucleic acid materials and toward the development of luminescent metal ions and complexes as sensors of RNA and DNA targets.

Janet R. Morrow
Christopher M. Andolina
Ryan A. Mathews
Brooke Switala

Chemistry Department,
Univ. at Buffalo, SUNY,
Amherst, NY 14260

phone: (716)645-6800-2152
fax: (716)645-6063
Email: jmorrow@buffalo.edu