Conversation 11: No. 2

category image Volume: Conversation 11
Issue Number 2
May 2000
ISBN 0-940030-81-0

An Earring for the Double Helix: Assembly of Topological Links Comprising Duplex DNA and a Circular Oligodeoxynucleotide

Novel DNA nanostructures, locked pseudorotaxane and locked catenane were assembled through topological linkage of a double-stranded target to a circular oligodeoxyribonucleotide (cODN)+. The formation of these supramolecular complexes occurs with remarkable sequence specificity and is accomplished via local opening of duplex DNA by a pair of homopyrimidine bis-PNAs. The obtained cODN label, resembling an earring, forms a true topological link with the linear or closed circular (cc) target DNA and occupies a fixed position along the double helix. The PNA directed assembly described here introduces PNA oligomers into the repertoire of DNA nanotechnological tools.

Heiko Kuhn
Vadim V. Demidov
and Maxim D. Frank-Kamenetskii

Center for Advanced Biotechnology
Department of Biomedical Engineering
Boston University
36 Cummington St.
Boston, MA 02215


Subscription is more cost effective than purchasing PDFs on-the-fly.  Click here for details.