Albany 2015:Book of Abstracts

Albany 2015
Conversation 19
June 9-13 2015
©Adenine Press (2012)

A Nanomechanical Device that Operates in a 3D Crystal

The central goals of structural DNA nanotechnology include both the self-assembly of matter in crystalline arrays and the construction of nano-scale devices. We have previously reported the construction of self-assembled 3D DNA crystals (Zheng et al., 2009) and the assembly of a variety of nano-scale DNA devices (Seeman, 2005). In addition, we have reported the combination of devices and arrays or DNA origami in two dimensions (Gu et al., 2010). Clearly, fulfillment of the goals of the field requires the extension of such systems to 3D. At this point, it is most convenient to build a device whose operation can be detected directly from the macroscopic crystals, rather than from their crystallographic structures. We have made this choice at the present time because robust nanomechanical devices will require large motions to be detectable, and the crystals available that allow such motions do not diffract well (Zheng et al., 2009). By contrast, changes of colored states induced by toehold strand replacement are readily visible at the macroscopic scale. Here we report a color-changing crystal, and its low-resolution crystal structure.

This research has been supported by he following grants to NCS: GM-29554 from NIGMS, grants CCF-1117210, CMMI-1120890, and EFRI-1332411 from the NSF, MURI W911NF-11-1-0024 from ARO, grants N000141110729 and N000140911118 from ONR, grant 3849 from the Gordon and Betty Moore Foundation.

    Zheng, J., Birktoft, J.J., Chen, Y., Wang, T., Sha, R., Constantinou, P.E., Ginell, S.L., Mao, C., Seeman, N.C.. From molecular to macroscopic via the rational design of a self-assembled 3D DNA crystal. Nature 461, 74-77 (2009).

    Seeman, N.C., From Genes to Machines: DNA Nanomechanical Devices, Trends Biochem. Sci. 30, 119-125 (2005).

    Gu, H., Chao, J., Xiao, S.J., Seeman, N.C., A Proximity-Based Programmable DNA Nanoscale Assembly Line, Nature 465, 202-205 (2010).

Yudong Hao1
Jens J. Birktoft, 1
Martin Kristiansen1
Ruojie Sha1
Chengde Mao 2
Nadrian C. Seeman1*

1 Department of Chemistry
New York University
New York, NY 10003, USA
2 Department of Chemistry
Purdue University
West Lafayette, IN, 47907 USA