Book of Abstracts: Albany 2009
June 16-20 2009
© Adenine Press (2008)
Wires, Reporters and Information Capsules: Cellular journalism with DNA
DNA has attractive physicochemical characteristics such as robust thermal and hydrolytic stability. It also has desirable structural characteristics stemming from predictable and specific recognition properties that give rise to a highly regular helical structure which behaves as a rigid rod on length scales upto ~50 nm. Since these rigid rods may be welded together by complementary base-pairing, DNA is now taking on a new aspect where it is finding use as a construction element for architecture on the nanoscale. This field is called structural DNA nanotechnology. I describe approaches adopted by my lab where we demonstrate promising new assembly strategies that use unusual forms of DNA in structural DNA nanotechnology to make chemically responsive DNA scaffolds. I will then go on to show the application of these chemically responsive DNA scaffolds in living systems.
References and Footnotes
- Ghodke, H. B.; Krishnan, R.; Vignesh, K.; Kumar, G.V.P.; Narayana, C.; Krishnan, Y.* The I-tetraplex building block: Rational Design and Controlled Fabrication of robust 1D DNA Scaffolds via non-Watson Crick self assembly. Angew. Chem. Int. Ed., 46, 2646-2649 (2007).
- Bhatia, D., Mehtab, S., Krishnan, R., Indi, S.S., Basu, A., Krishnan, Y.* Icosahedral DNA nanocapsules via modular assembly. Angew. Chem. Int. Ed. in press.
- Modi, S., Swetha M. G., Goswami, D., Gupta, G., Mayor, S., Krishnan, Y.* A DNA nanomachine maps spatiotemporal pH changes in living cells. Nature Nanotechnology, accepted.
- Chakraborty, S., Sharma, S., Maiti, P., Krishnan, Y.* The poly dA helix: A new structural motif for high-performance DNA-based molecular switches. Nucleic Acids Res., in press.
The Chemical Biology Group
National Centre for Biological Sciences, TIFR
UAS-GKVK Campus, Bellary Road
Bangalore 560 065,
email Yamuna Krishnan
Yamuna Krishnan received a PhD in Organic Chemistry (2002) from IISc, Bangalore. She then went on pursue her postdoctoral studies as an 1851 Research Fellow at the University of Cambridge (UK) and later elected to the Fellowship of Wolfson College, where she explored the materials properties of quadruplex DNA. In 2005 she returned to set up her own group at the NCBS, TIFR, Bangalore where her group develops nucleic acid assemblies to interrogate cellular processes. She is an Associate of the Indian Academy of Sciences and a recipient of the the Innovative Young Biotechnologist Award, DBT, GoI.