Albany 2013: Book of Abstracts

category image Albany 2013
Conversation 18
June 11-15 2013
©Adenine Press (2012)

Vascular Endothelial Growth Factor Receptor-2 (VEGFR2): Structure and Functional relationship

Vascular endothelial growth factor, VEGF, is expressed in the vicinity of sprouting vessels, and its receptor (VEGF-R2/Flk-1/kdr) on the angioblasts and new vessels, and both are required for vasculogenesis and angiogenesis. VEGFR2, also called as KDR or Flk-1, is identified as an early marker for endothelial cell progenitors, whose expression is restricted to endothelial cells in vivo. VEGFR2 consists of extracellular (7-Ig- like sub-domains), transmembrane and cytoplasmic domains. In order to understand the structure-functional relationship and signal transduction process of VEGFR2, we have examined their aminoacid sequences from a wide range of species including mammals, birds, Zebrafish and also computed the phylogenetic tree, secondary and domain structures. Phylogeny constructed using Maximum Parsimony tree software MEGA-5 version suggested an interesting sequence similarity between Zebrafish and Gallus, closeness between human, rat, horse and pig. Strong homology in aminoacids sequences were observed between the species human, Macaca mulatta, gorilla etc; and small variations in Zebrafish and zebrafinch. The Arg and Asp residues which are involved in forming salt bridges are evolutionarily conserved from Zebrafish to human in D7 domain of VEGFR2, indicating their functional importance in VEGFR activity. Aminoacids tyrosine in the extracellular loops and cysteines involved in disulphide bridges of VEGFR2 are highly conserved suggesting their importance during ligand binding, the details of which will be discussed.


    Carmeliet, P., Ferreira, V., Breier, G., Pollefeyt, S., Kieckens, L., Gertsenstein, M., Fahrig, M., Vandenhoeck, A., Harpal, K., Eberhardt, C. et al. (1996). Abnormal blood vessel development and lethality in embryos lacking a single VEGF allele. Nature380, 435-439.

    Leung, D., Cachianes, G., Kuang, W. J., Goeddel, D. V. and Ferrara, N. (1989). Vascular endothelial growth factor is a secreted angiogenic mitogen. Science 246,1306-1309.

    Shalaby, F., Rossant, J., Yamaguchi, T. P., Gertsenstein, M., Wu, X. F., Breitman, M. L. and Schuh, A. C. (1995). Failure of blood-island formation and vasculogenesis in flk-1 deficient mice. Nature 376, 62-66.

Jhansi Rani Nathan
Shilpi Shika
Malathi Ragunathan*

Department of Genetics
University of Madras
Taramani, Chennai- 600 113, India

*Ph: +91-44-24547062
Fx: +91-44-24540709