Albany 2015:Book of Abstracts
June 9-13 2015
©Adenine Press (2012)
Two dimensional nanomaterials for microRNA analysis
Targeting, silencing or imaging miRNA molecules have been considered promising methods for fighting cancer at the molecular level. microRNAs (miRNAs) are small noncoding RNA molecules (≈22 nucleotides) that regulate gene expression at the post-transcriptional level. miR-10b has been found over-expressed in metastatic breast cancer. Inhibiting the functions of miR-10b using single stranded synthetic anti-sense Locked Nucleic Acid (LNA) molecules has shown effective therapeutic results for metastatic breast cancer. The objective of this study was to understand the interaction between miR-10b with its complementary natural or unnatural anti-sense strands using two-dimensional graphene (nGO) and Molybdenum disulfide (MoS2). Here we used nGO and MoS2 to investigate the interaction between an LNA modified antisense oligonucleotide and its target oncomiR (miR-10b). In this study, nGO and MoS2 serve as a firm nano-platform for nucleic acids with or without base modification, while providing rich kinetic and spectroscopic information about their interaction. Moreover, we were able to validate the stability of the oligonucleotide and nGO or MoS2 nanoassemblies (nGO/ssDNA or MoS2/ssDNA) at different temperatures. In conclusion, we demonstrate that two-dimensional nanomaterials serve as a superb nano-platform for monitoring nucleic acid interactions, which have essential implications in miRNA imaging and therapeutic technologies. We were able to observe the similarities and differences in interactions between miR-10b and its LNA or DNA complementary strands using rapid quenching and adsorbing properties of nGO and MoS2. The stability of the nGO/ssDNA or MoS2/ssDNA nanoassemblies in a wide range of temperatures suggests that it is an attractive nanomaterial for monitoring hybridization and de-hybridization of oligonucleotides.
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1Department of Chemistry