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Albany 2015:Book of Abstracts

Albany 2015
Conversation 19
June 9-13 2015
©Adenine Press (2012)

Two different hormone transporting systems in human blood Features of peptide hormone transport in human blood

It is well known, that serum transport proteins take part in hormone delivering to the sensitive tissues. However, according to many researchers, along with this mechanism, hormones are transporting and depositing by erythrocytes (Sundulyak, 1974; Osty et.al., 1988; Dolomatov, 1999, Eliseeva, 2009).

Ratio of hormone transport by erythrocytes and by serum in human blood didnot get experimental estimation yet. The aim of this research is to define this ratio for peptide hormones. The ratio was determined as the value of the coefficients of hormone distribution between the erythrocytes surface and serum.

100 blood samples of healthy men 25-35 years were tested. Coefficient was calculated as ratio of prolactin, insulin, luteinizing, foliculostimulant, thyrotrophic hormone concentrations in samples, eluted from erythrocytes surface to it's concentration in serum. Concentrations were determined by means of ELISA. Results are exposed in the table.

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The obtained data show, that all investigated protein hormones, except insulin, are transporting mainly by erythrocytes. The values of peptides hormones distribution coefficients, except insulin, are more than 100. Therefore, most of protein hormones deposits and are delivered to tissues mainly by erythrocytes. It's shown, that along with the transfer of the protein hormone in complex with the erythrocytes surface receptors, transport of protein hormones in human blood is also due to their saturation in the internal erythrocytes volume. For example, adrenotropic hormone concentration in healthy donors hemolisates was equal to 247, 8 ±82 pg/ml and was much more stable concerning to it's concentration in serum.

From literature it's known, that hydrophobic hormones are delivering by serum transport proteins and by erythrocytes too (Dolomatov, 1999). Thus, in human blood there are two hormone delivering systems: serum proteins and erythrocytes that are universal transporting cells. Obtained data suggest, that protein hormones are transporting mainly by erythrocytes. Insulin is an exception from this rule. Coefficient of it's distribution equal to 0,49±0,016. Therefore, insulin, in contrast to other protein hormones, is delivering to tissues in equal participation of both transporting systems. Obtained data show, that insulin reacts with serum transporting proteins, which is consistent with previously obtained data (Garipova 2013).

References
    Sundulyak L.I. (1974). Erythrocytes as a depo and insulin transporting system. DOKL. Akad.Nauk SSSR., 219, 4, 1020-1021.

    Osty J., Laurence J., Jacques F., Blondeau J.-P. ( 1988). Tiroksin-binding erythrocyte function. Endocrinology, 123 , N 5, 2303-2311.

    Dolomatov S.I., Pishak V. P., Slipenuk T. S., Meshishen I. F., Okopnaya T.V. (1999). Erythrocyte capacity to depose thyroid hormones: regulatory function of physicochemical factors in vitro. Voprosy Med. Chimii, 6, 572-577.

    Eliseeva O. S., Kireeva N. A., Pershina A. S., Garipova M.I. (2009). Investigation of the insulin interaction with the erythrocytes surface. Vestnik OGU, N 6, 476-478.

    Garipova M.I., Usmanova R.R. (2013). Isolation and partial characterization of a general hormone transporting blood protein complex. Journal of Biomolecular Structure and Dynamics, 31, S1, 118.


Margarita I. Garipova
Sergey A. Bashkatov
Olesya I. Dazko
Anna A. Kosova
Maria E. Migunova

Bashkirian State University
450076, Ufa,
Z. Validy, 32 Russia Ph: (347)2299671
Fax: (347)273-67-78
margaritag@list.ru