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Conversation 11: No. 2

category image Volume: Conversation 11
Issue Number 2
May 2000
ISBN 0-940030-81-0

The Sensitivity of the B-Z Transition of DNA to Multivalent Cations in High and Low Concentrations: A Unified Electrostatic Interpretation

We showed recently that the high-salt transition of poly[d(G-C)]·poly[d(G-C)] between B-DNA and Z-DNA (at [NaCl] = 2.25 M or [MgCl2] = 0.7 M) can be ascribed to the lesser electrostatic free energy of the B form, due to better immersion of the phosphates in the solution. This property was incorporated in cylindrical models of B-DNA and Z-DNA which were analyzed by Poisson-Boltzmann theory. The results are insensitive to details of the models, and in fair agreement with experiment. In contrast, the Z form of the poly[d(G-m55C)] duplex is stabilized by very small concentrations of magnesium. We now show that this phenomenon is easily explained by the same electrostatic theory, without any adjustable parameter. The very different responses to magnesium of the methylated and non-methylated polymers stem from a modest and salt-independent change in the non-electrostatic component of the free energy difference between the Z and B forms. This does not involve any stereo-specific interaction between DNA and the cation. The theory also explains quantitatively the effect of micromolar concentrations of trivalent cobalt hexammine on the B-Z transition, and it provides a framework for describing the influence of temperature and of solvent changes. The difference between the effect of alkaline-earth and transition metal ions on the transition of poly[d(G-C)] is explained by metal coordination (e.g. to guanine N7). This ion-specificity requires only a modest affinity. Hence, in the case of the B-Z transition as in others (e.g. the folding of tRNA and of ribozymes), the effect of multivalent cations on nucleic acid structure is mediated primarily by electrostatic, non-specific interactions. We propose this as a general rule for which convincing counter-examples are lacking.

J.-Ph. Demaret1
and M. Guéron2

1L.P.B.C.
Université Pierre et Marie Curie
4 place Jussieu
75252 Paris cedex 05, France
2Groupe de biophysique de l?Ecole polytechnique et de l?UMR 7643 du CNRS
Ecole polytechnique
91128 Palaiseau, France

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