Book of Abstracts: Albany 2003

category image Albany 2003
Conversation 13
Abstract Book
June 17-21 2003

The Fluctuating Enzymes

Our single-molecule experiments have shown that an enzyme is a dynamical entity whose conformation and enzymatic rate undergo constant fluctuation over a broad range of time scales. The phenomenon has been observed in several systems: cholesterol oxidase, flavin reductase, and exo-nuclease. Various single-molecule experiments, such as observing enzymatic turnovers by fluorescence, probing conformational dynamics by photoinduced electron transfer, and studying DNA/enzyme interaction by a simple flow assay, have led to new insights into the fluctuating enzymes. The latest results will be presented.

Sunney Xie

Department of Chemistry and Chemical Biology
Harvard University
12 Oxford Street M026
Cambridge, MA 02138