Book of Abstracts: Albany 2003
June 17-21 2003
The Fluctuating Enzymes
Our single-molecule experiments have shown that an enzyme is a dynamical entity whose conformation and enzymatic rate undergo constant fluctuation over a broad range of time scales. The phenomenon has been observed in several systems: cholesterol oxidase, flavin reductase, and exo-nuclease. Various single-molecule experiments, such as observing enzymatic turnovers by fluorescence, probing conformational dynamics by photoinduced electron transfer, and studying DNA/enzyme interaction by a simple flow assay, have led to new insights into the fluctuating enzymes. The latest results will be presented.
Department of Chemistry and Chemical Biology