Albany 2013: Book of Abstracts
June 11-15 2013
©Adenine Press (2012)
The ESCRT Pathway in HIV Budding and Cell Division
The Endosomal Sorting Complexes Required for Transport (ESCRT) pathway mediates membrane fission reactions during intraluminal endosomal vesicle formation, budding of HIV-1 and other enveloped viruses, and the final abscission step of cytokinesis in mammals and archaea. Current models hold that ubiquitin-binding ESCRT factors act early in the pathway to regulate factor recruitment and assembly, whereas the late-acting ESCRT-III proteins form filaments that draw the membranes together and mediate fission, possibly in collaboration with VPS4 ATPases.
I will discuss our current understanding of the structures and functions of the different ESCRT factors in HIV budding and abscission, with a particular focus on our studies aimed at understanding: 1) how ubiquitin regulates ESCRT recruitment during HIV-1 budding, and 2) the structures and membrane binding properties of ESCRT-III subunits and filaments.
Wesley I. Sundquist
Department of Biochemistry