Albany 2015:Book of Abstracts

Albany 2015
Conversation 19
June 9-13 2015
©Adenine Press (2012)

The busy life of nascent chains: Mechanisms of folding of newly synthesized proteins

Protein homeostasis is established by a complex cellular machinery which assists and regulates regular folding pathways and counteracts protein misfolding and aggregation. A particularly critical process in the life of a protein is the native folding of newly synthesized proteins, which therefore is tightly controlled. Already during ongoing synthesis by the ribosome nascent polypeptides are subject to enzymatic processing, chaperone-assisted folding to the native state or targeting to translocation pores at membranes. The ribosome itself plays a key role in these different tasks by serving as platform for the regulated association of enzymes, targeting factors and chaperones that act upon the nascent polypeptides emerging from the exit tunnel. The molecular mechanisms integrating the different co-translational processes leading to the maturation and native folding of nascent chains will be described.

    G. Kramer, G., Boehringer, D., Ban, N. & Bukau, B. (2009). The ribosome as a platform for co-translational processing, folding and targeting of newly synthesized proteins. Nat Struct Mol Biol 16, 589-597.

    F. Gloge, F., Becker, A.H., Kramer, G. & Bukau, B. (2014). Co-translational mechanisms of protein maturation. Curr Opin Struct Biol 24, 24-33.

    A. Hoffmann, A., Becker, A.H., Zachmann-Brand, B., Deuerling, E., Bukau, B. & Kramer, G. (2012). Concerted action of the ribosome and the associated chaperone trigger factor confines nascent polypeptide folding. Mol Cell 48, 1-12.

    E. Oh, E., Becker, A.H., Sandikci, A., Huber, D., Chaba, R., Gloge, F., Nichols, R.J., Typas, A., Gross, C.A., Kramer, G., Weissman, J.S. & Bukau, B. (2011). Selective ribosome profiling reveals the cotranslational chaperone action of trigger factor in vivo. Cell 147, 1295-1308.

    A. Mashaghi, A., Kramer, G., Bechtluft, P., Zachmann-Brand, B., Driessen, A. J., Bukau, B. & Tans, S. J. (2013). Reshaping of the conformational search of a protein by the chaperone trigger factor. Nature 500, 98-101.

Guenter Kramer
Bernd Bukau

Center for Molecular Biology of the University of Heidelberg
German Cancer Research Center
DKFZ-ZMBH Alliance
Im Neuenheimer Feld 282
D-69120 Heidelberg, Germany