Telomerase Activity, Expression and Telomere in situ Analysis in the Course of Treatment of Childhood Leukemias
Childhood leukemia samples of peripheral blood and bone marrow were tested for telomerase activity and expression in the day of diagnosis and in the course of therapy. A tight correlation with clinical response was observed in most patients: most of patients showed a complete morphological remission and corresponding decrease of telomerase activity almost to the levels of healthy donors within several days after diagnosis. A unique case was observed in which telomerase activity was only moderately increased on diagnosis, it gradually increased in the course of therapy, and only after application of intensified therapy a subsequent decrease has occurred. This patient did not respond to therapy, his disease progressed and he finally died during intensified therapy. In another patient, analysis of telomere lengths using a dideoxy-PRINS revealed a single telomere expansion at chromosome IV.
Supported by the Grant Agency of the Czech Republic (projects no. 301/99/0045 and 204/00/P012) and the Grant Agency of the Academy of Sciences of the Czech Republic (project no. S5004010)
J. Malaskaa,b, M. Sklenickovaa, K. Krejcia,c,M. Bajerb, H. Hrstkovab, L. Fajkusovaa,d, and J. Fajkusa,c,
aDepartment of Analysis of Biologically Important Molecular Complexes,