Albany 2013: Book of Abstracts
June 11-15 2013
©Adenine Press (2012)
T-cell vaccine design for Streptococcus pneumoniae: an in silico approach
Streptococcus pneumoniae (pneumococcus) remains an important cause of meningitis, bacteremia, acute otitis media, community acquired pneumonia associated with significant morbidity and mortality in world wide. Conjugated polysaccharide, glycoconjugated and capsular polysaccharide based vaccines were existent for pneumococcal disease but still are specific and restricted to serotypes of Streptococcus pneumoniae. Proteome of eight serotypes of Streptococcus pneumoniae was retrieved and identified common proteins (Munikumar et al., 2012a). 18 membrane proteins were distinguished from 1,657 common proteins of eight serotypes of Streptococcus pneumoniae. Implementing comparative genomic approach and subtractive genomic approach three membrane proteins were predicted as essential for bacterial survival and non-homologous to human (Umamaheswari et al., 2011, Munikumar et al., 2012a). ProPred server was used to propose four promiscuous T-cell epitopes from three membrane proteins and validated through published positive control, SYFPEITHI and immune epitope database (Munikumar et al., 2012b). The four epitopes docked into peptide binding region of predominant HLA-DRB alleles with good binding affinity in Maestro v9.2. The T-cell epitope 89-VVYLLPILI-97 and HLA-DRB5*0101 docking complex was with best XPG score (-13.143 kcal/mol). Further, the stability of the complex was checked through molecular dynamics simulations in Desmond v3.3. The simulations results had revealed that the complex was stable throughout 5000 ps (Munikumar et al., 2012b). Thus, the epitope would be ideal candidate for T-cell driven subunit vaccine design against selected serotypes of Streptococcus pneumoniae.
This research was supported by DBT, Ministry of Science & Technology, BIF program, Govt. of India (No. BT/BI/25/001/2006).
M. Munikumar, M., Priyadarshini, V., Pradhan, D., Umamaheswari, A., & Vengamma, B. (2012b) Computational approaches to identify common subunit vaccine candidates against bacterial meningitis. Interdisciplinary Sciences: Computational Life Sciences (In press).
A. Umamaheswari, A., Pradhan, D., & Hemanthkumar, M. (2011) Computer aided subunit vaccine design against pathogenic Leptospira serovars. Interdisciplinary Sciences: Computational Life Sciences 4:38-45.