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Book of Abstracts: Albany 2005

category image Volume 22
No. 6
June 2005

Structure and Mechanism of RecBCD

Double strand breaks in bacterial cells can result from a variety of things including collapsed replication forks or other DNA damage. One mechanism for repair of breaks involves the multifunctional enzyme complex, RecBCD. RecBCD comprises two distinct DNA helicase subunits, a number of differentially regulated nuclease activities, and the ability to recognize a recombinational hotspot called Chi. In order to understand more about the molecular basis of these activities we have determined the crystal structure of RecBCD complexed with DNA. The structure reveals the basis for the two different helicase activities and explains the regulation of nuclease digestion. The structure also suggests how the enzyme might be able to scan DNA for Chi sequences as the DNA passes through the protein complex.

References and Footnotes
  1. Singleton, M. R., Dillingham, M. S., Gaudier, M., Kowalczykowski, S. C. and Wigley, D. B. Nature 432, 187-193 (2004).

Martin Singleton
Mark Dillingham
Martin Gaudier
Steve Kowalczykowski
Dale B. Wigley*

Cancer Research UK Clare Hall Laboratories
South Mimms
Potters Bar
Herts EN6 3LD, U.K.

*Phone: +44 -(0)207 269 3930
Fax: +44 -(0)207 269 3803
Email: Dale.Wigley@cancer.org.uk