Book of Abstracts: Albany 2003

category image Albany 2003
Conversation 13
Abstract Book
June 17-21 2003

Structural DNA Nanotechnology

DNA nanotechnology uses reciprocal exchange between DNA double helices or hairpins to produce branched DNA motifs, like Holliday junctions, or related structures, such as double crossover (DX), triple crossover (TX), paranemic crossover (PX) and DNA parallelogram motifs. We combine DNA motifs to produce specific structures by using sticky-ended (below, left) or PX or edge-sharing cohesion. From simple branched junctions, we have constructed DNA stick-polyhedra, such as a cube (below, right) and a truncated octahedron, several designed knots, and Borromean rings. We have used two DX molecules to construct a DNA nanomechanical device by linking them with a segment that can be switched between Z-DNA and B-DNA. PX DNA has been used to produce a robust sequence-dependent device that changes states by varied hybridization topology.

A central goal of DNA nanotechnology is the self-assembly of periodic matter. We have constructed micron-sized 2-dimensional DNA arrays from DX, TX and parallelogram motifs. We can produce specific designed patterns visible in the AFM from DX and TX molecules. We can change the patterns by changing the components, and by modification after assembly. In addition, we have generated 2D arrays with tunable cavities from DNA parallelograms. In studies complementary to specific periodic self-assembly, we have performed algorithmic constructions, corresponding to XOR operations.

The key challenge in the area is the extension of the 2D results obtained so far to 3D systems. We expect to be able to produce high resolution crystals of DNA host lattices with heterologous guests, leading to well-ordered bio-macromolecular systems amenable to diffraction analysis. Other challenges are to incorporate DNA nanomechanical devices in periodic and aperiodic lattices and to use the lattices to organize nanoelectronic components. The existence of living systems with nanoscale structural components represents an existence proof that autonomous systems can build up and function on this scale, systems capable of energy transduction and replication. The overall challenge that biology presents to the physical sciences is to move from biokleptic to biomimetic to abiological systems that perform in this same manner.

This research supported by NIGMS, ONR, DARPA/AFOSR, and NSF.

Nadrian C. Seeman*
Hao Yan
Chengde Mao
Ruojie Sha
Furong Liu
Xiaoping Zhang
Zhiyong Shen
Pamela E. Constantinou
Baoquan Ding
Wanqiu Shen
Shiping Liao
Lisa Israel
Philip Lukeman
Yariv Pinto
William Sherman
Jiwen Zheng
Jens Kopatsch
Loraine Foley
Xiaoping Yang
Weiqiong Sun
Yinli Wang
Shouming Du
Hui Wang
Tsu-Ju Fu
Yuwen Zhang
Hangxia Qiu
John E. Mueller
Junghuei Chen

Department of Chemistry
New York University
New York, NY 10003, USA