Book of Abstracts: Albany 2009
June 16-20 2009
© Adenine Press (2008)
Structural and Mechanistic Insights into Single-stranded DNA Transposition
DNA transposition is a process in which discrete segments of DNA are moved from one genomic location to another, accomplished through a series of DNA strand cutting and joining reactions catalyzed by a transposase. There are a surprisingly large number of mechanistically different ways how DNA transposition is carried out and regulated. Our recent mechanistic and structural studies into the transposition pathway of IS608, an insertion sequence originally identified in Helicobacter pylori, have provided the first insights into a pathway that acts asymmetrically on single-stranded DNA.
IS608 always inserts just 3' of a TTAC tetranucleotide. We have established that the mode of target site recognition relies on interactions between an internal sequence of the transposon and the target sequence, opening up an unexpected approach to site-specific targeting of transposition. We have recently demonstrated that we can direct insertions in a predictable way into a variety of chosen target sequences, both in vitro and in vivo.
1Laboratory of Molecular Biology