19th-banner-rev.gif

Book of Abstracts: Albany 2003

category image Albany 2003
Conversation 13
Abstract Book
June 17-21 2003

Some Characteristics of DNA Melting Curve

The method of definition of average values describing distribution of nucleotides along DNA strands is supposed through decomposition of a differential melting curve (DMC) of DNA on components set by Gauss`s function.

For approximation of DMC, superposition of Gauss`s functions is conveniently used

where Sl, σl, Tl accordingly are area, root-mean-square width and position on a temperature scale of a component l.

Let`s calculate first and central moments of the maximum orders for DMC of DNA
Yu. S. Babayan1
A. Sh. Markaryan1
S. Yu. Babayan1
M. A. Parsadanyan2
A. P. Antonyan2
P. O. Vardevanyan2

1Molecular Physics Department
Yerevan State University
375025 Yerevan, Armenia 2Biophysics Department of Yerevan State University
375025 Yerevan, Armenia
*Phone (3741) 571 061
simvar@arminco.com


M1 characterizes DNA melting temperature, M2 determines a root-mean-square deviation by the formula σ=(M2)1/2 and is connected to DNA melting interval. M3 characterizes the asymmetry of DMC through parameter Ac=M33: Ac>0, if DMC is shifted in the range of high temperatures relative M1. M4 characterizes a sharpness of DMC in relation to Gauss`s, if parameter

The accounts show, that the values of parameters M, σ, Ac and Ek are steady on a choice of a decomposition mode of DMC and within the limits of definition error of experimental data coincide. It indicate that through decomposition of DNA DMC it is possible to calculate the integral characteristics of a DNA melting curve.

Yu. S. Babayan1
A. Sh. Markaryan1
S. Yu. Babayan1
M. A. Parsadanyan2
A. P. Antonyan2
P. O. Vardevanyan2

References and Footnotes
  1. Vardevanyan P. O., Babayan Yu. S., Vardapetyan R. R., Panosyan G. H., Aslanyan V. M., Karapetyan A. T. Biofizika (Rus.), 1983, v.28, N1, p.130-132.