Albany 2015:Book of Abstracts
June 9-13 2015
©Adenine Press (2012)
Smart polymer Functionalized Graphene Nano-Devices for Thermo-Switch Controlled Biodetection
In this work, we have developed a general methodology for constructing an activatable biosensor utilizing a thermo-responsive polymer (Yavuz et al. 2009) and two-dimensional nano-sheet. We have demonstrated the detection of four different types of biological compounds using the smart PEGMA (poly(ethylene glycol) methyl ether methacrylate), oligonucleotides and graphene oxide nano-assembly. The activity of the functional nano-device is controlled with a thermo-switch at 39 °C. In this design, the nano-sized graphene oxide serves as a template for fluorophore labeled probe oligonucleotides while quenching the fluorescence intensities dramatically (Rana et al. 2014). On the other hand, the PEGMA polymer serves as an activatable protecting layer covering the graphene oxide and entrapping the probe oligonucleotides on the surface. The PEGMA polymers are hydrophobic above their lower critical solution temperature (LCST) and therefore interact strongly with the hydrophobic surface of graphene oxide, creating a closed configuration (OFF state) of the nano-device. However, once the temperature decreases below the LCST, the polymer undergoes conformational change and becomes hydrophilic. This opens up the surface of the graphene oxide (open configuration, ON state), freeing the encapsulated payload on the surface. We have tuned the activity of the nano-device for the detection of a sequence specific DNA, miR-10b, thrombin and adenosine. The activity of our functional system can be decreased by ~80% with a thermo-switch at 39 °C. Our approach can be extended to other anti-sense oligonucleotide, aptamer or DNAzyme based sensing strategies.
M. Rana, M. Balcioglu, N. Robertson and M. V. Yigit. (2014) Nano-graphene oxide as a novel platform for monitoring the effect of LNA modification in nucleic acid interactions. Analyst. 139, 714-720.
Mustafa Balcioglu 1
1 Department of Chemistry