Book of Abstracts: Albany 2005
Regulation of Gene Expression with Programmable DNA Binding Oligomers
DNA binding polyamides are synthetic oligomers which mimic regulatory proteins and can be programmed to bind a large repertoire of DNA sequences (1, 2). Modified polyamides are cell permeable and shown to traffic to the nucleus (3). The integration of polyamides into the transcriptional machinery of living cells to control biological circuitry can be imagined by three different strategies. Interfere with regulatory proteins in the promoter of endogenous genes can result in gene silencing (4). Recruitment of the transcriptional machinery in promoters of specific genes can activate gene expression (5, 6). Target the coding region of genes by a covalent steric blockade inhibits polymerase elongation (7).
References and Footnotes
Peter B. Dervan
Division of Chemistry & Chemical Engineering