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Conversation 11: No. 2

category image Volume: Conversation 11
Issue Number 2
May 2000
ISBN 0-940030-81-0

Recent Highlights from Atomic Force Microscopy of DNA

Seven recent highlights are presented from atomic force microscopy (AFM) of DNA in this lab. The first two involve advances in the observation of enzymatic reactions in near-physiological solutions. E. coliRNA polymerase was observed to process along its DNA template in a series of time-lapse images [S. Kasas, et al., Biochemistry 36, 461 (1997)], and a new small-cantilever atomic force microscope (AFM) imaged DNA degradation by DNase I at rates as fast as two seconds per image. The next five highlights involve structural observations of DNA and DNA-protein complexes, including DNA condensed for gene delivery, sequence-dependent DNA condensation, an AFM assay for RNA polymerase, and AFM evidence for a yeast kinetochore complex that may be involved in holding together sister chromatids during cell division.

Helen G. Hansma1
Lía I. Pietrasanta1
Roxana Golan1
John C. Sitko1
Mario B. Viani1
George T. Paloczi1
Bettye L. Smith1
Douglas Thrower1,3
and Paul K. Hansma1

1Department of Physics
University of California
Santa Barbara, CA 93106
2Department of Biological Sciences
University of California
Santa Barbara, CA 93106
3Current address is:
Department of Biology
University of North Carolina
Chapel Hill, NC 27599-3280

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