Albany 2013: Book of Abstracts
June 11-15 2013
©Adenine Press (2012)
Porous three-dimensional DNA crystals as biomolecular containers for catalysis
The major goal of DNA nanotechnology has been the design and manufacture of artificial DNA structures for technological uses. The porous three-dimensional DNA crystals have been proposed as macromolecular scaffolds for host-guest structure determination, as molecular sieves, and as molecular containers for catalysis. By using fluorescence dequenching technique we have demonstrated that a protein enzyme adsorbed in a designed three-dimensional DNA crystal is capable of performing catalysis. The axially distinct aperture sizes in the crystal design allowed us to improve the enclosing of the enzyme with a protective protein-based “coating” cross-linked over the crystal surface. This coating allows entry and exit of small molecules through the crystal while restricting enzymes inside the crystal. This enzyme-enclosed DNA crystal is capable of performing multiple cycles of catalysis and it retained its enzymatic activity over numerous days after being protein-coated. The concepts of the enzyme-enclosed DNA crystal and the unique protein coating technique provide possibilities to the development of enzyme replacement therapies and biodegradable solid-state catalysts and biosensors.
This research has been supported by NSF CAREER award DMR-1149665
Paukstelis, P. J. Three-dimensional DNA crystals as molecular sieves. J Am Chem Soc 128, 6794-6795 (2006).
Center for Biomolecular Structure and Organization