Albany 2013: Book of Abstracts
June 11-15 2013
©Adenine Press (2012)
Optimizing DNA Nanotube Design for Future Applications
DNA nanotubes hold promise as scaffolds for protein organization, as templates of nanowires and photonic systems, and as drug delivery vehicles (Cao 2004). We present a new DNA-economic strategy for the construction of DNA nanotubes with a backbone produced by rolling circle amplification (RCA), which results in increased stability and templated length (Hamblin, et al. 2012). These nanotubes are more resistant to nuclease degradation, capable of entering human cervical cancer (HeLa) cells with significantly increased uptake over double-stranded DNA, and are amenable to encapsulation and release behavior. As such, they represent a potentially unique platform for the development of cell probes, drug delivery, and imaging tools. In a second iteration, we also present a modified design that has been simplified even further (Hamblin, et al. 2013). This allows rapid, room temperature assembly of high aspect ratio nanotubes from just five unmodified DNA strands and the RCA backbone.
This research has been supported by NSERC & CSACS.
G. D. Hamblin, K. M. Carneiro, J. F. Fakhoury, K. E. Bujold, & H. F. Sleiman. (2012). Rolling circle amplification-templated DNA nanotubes show increased stability and cell penetration ability. J. Am. Chem. Soc. 134(6), 2888-2891.
G. D. Hamblin, A. Hariri, K. M. Carneiro, K. L. Lau, G. Cosa, & H. Sleiman. (2013). A Simple Design for DNA Nanotubes from a Minimal Set of Unmodified Strands: Rapid, Room Temperature Assembly and Readily Tunable Structure. ACS Nano, submitted.
Graham D. Hamblin
Department of Chemistry