Albany 2013: Book of Abstracts

category image Albany 2013
Conversation 18
June 11-15 2013
©Adenine Press (2012)

Novel Methods to Identify and Quantify Protein Kinetic stability in different Systems

Kinetically stable proteins (KSPs) refer to proteins that are trapped by an energy barrier in a specific state, unable to transiently sample other conformations. Kinetic stability (KS) may be a feature used by nature to protect the native structure of a protein against misfolding or harsh conditions. The biological and pathological significance of KS remains poorly understood, in part due to the lack of simple experimental methods to identify this property at a proteomics level. Based on our previously discovered correlation between KS and a protein’s resistance to sodium dodecyl sulfate (SDS), we show here three electrophoreses methods for identifying KSPs in complex mixtures using proteomics analysis or for quantifying the KS of proteins. We have applied these methods on diverse biological systems, including microorganisms, human plasma, and beans. Novel insight is emerging about the biological and pathological significance of this intriguing biophysical property of certain proteins.

This research has been supported by NSF Award 1158375


    Xia, K., Manning, M., Zhang, S., Colon, W. Proteomics analysis of kinetically stable proteins. Integrative Proteomics, page 281-300, ISBN 978-953-51-0070-6, InTech, Feb, 2012

    Xia, K, Zhang, S., Bathrick, B., Liu, S., Garcia, Y., Colon, W. (2012) Quantifying the kinetic stability of proteins via time-dependent SDS-trapping. Biochemistry 2012 Jan 10;51(1):100-7.

    Xia, K., Zhang, S., Solina, B., Barquera, B., Colón, W. Do prokaryotes have more kinetically stable proteins than eukaryotic organisms? Biochemistry. 2010, 49 (34), 7239–7241.

    Zhang, S., Xia, K., Chung, W. K., Cramer, S. M., Colón, W. Identifying kinetically stable proteins with capillary electrophoresis. Protein Science. 2010, 19(4):888-892.

    Xia, K., Manning, M., Hesham, H., Bystroff, C., Lin, Q., Colón, W. Identifying the subproteome of kinetically stable proteins via diagonal 2D SDS-PAGE. Proc. Natl. Acad. Sci., 2007, 104, 17329-17334.

Ke Xia
Wilfredo Colón

Department of Chemistry and Chemical Biology
Center for Biotechnology and Interdisciplinary Studies
Rensselaer Polytechnic Institute
Troy NY

Ph: (518) 276-2515