SUNY at Albany
June 19-23, 2001
New Approaches in Studying RNA-polymerase?Promoter Recognition Code
Using original method (1), calculation and analysis of electrostatic potential distribution around DNA fragments containing early T7 and T4 phage promoters was undertaken. These promoters can be classified into several groups differing by their sets of specific elements in far upstream regions of promoter DNA. It is interesting that this classification correlates well with specific response of the different promoter groups to some mutational changes in -subunit and ADP-ribosylation of -subunit of E.coli RNA polymerase (2,3). The data obtained indicate that electrostatic interactions may be important in differential recognition of various promoters by the enzyme. Electrostatic characteristics of DNA in upstream regions of promoters can be a new promoter determinant contributing to their strengths. What is more, this factor may be responsible for modulating promoter activities in response to some RNA polymerase modifications changing its electrostatic properties.
This work was supportted by the Russian Foundation for Basic Research (grant 99-04-48177)
References and Footnotes
* T.R. Dzhelyadin(1), A.A. Sorokin(1), V.S. Sivozhelezov(1), N.N. Ivanova(1), R.V. Polozov(2), S.G. Kamzolova(1)
(1)Institute of Cell Biophysics of RAS,