Mendel-Brno 2000

category image Volume: 17
Issue Number 6, Part 2
June 2000

Mutagenic of cyanobacterial bloom in Sulejow, Jeziorsko and Wloclawek reservoirs.

Toxins produced by species present in phytoplankton bloom threaten health of both people and animals not only due to acute toxicity of some of these compounds but because of their influence on cell genetic aparatus as well. Potential mutagenicity of cyanobacterial bloom extracts from Sulejow, Jeziorsko and Wloclawek were invesigated. Two test systems were used: in vivo root tip cells of Vicia faba subsp. minor and in vitro cultured human lymphocytes. Cyanobacterial extracts induced chromosomal aberrations and micronuclei in Vicia root meristems and human lymphocytes. The dominant types of aberrations were chromatid and chromosomal breaks, although some chromatid exchanges and dicentric chromosomes were also present. After treatment with cyanobacterial extracts mitodepressive effects and disturbances in mitosis were observed (chromatid bridges, lagging/lost chromosomes). The budding nuclei and two-nuclear cells were also observed. Our results indicate that cyanobacterial toxins have clastogenic, turbogenic and cytotoxic effects.

Renata Kontek, Regina Osiecka, Wanda Bratkowska1, Tomasz Ferenc1 Alina Blaszczyk, Anna Mordalska1, Dobroslawa Lopaczynska1, Malgorzata Tarczynska2, Henryk Strozynski3, Piotr Luchniak, Maciej Zalewski2

Department of Cytogenetics and Plant Molecular Biology,
University of Lodz, Poland
1Department of Biology and Medical Genetics,
Military Medical Academy of Lodz, Poland
2Department of Applied Ecology, University of Lodz, Poland
3Department of Infectious Diseases and Gastroenterology,
Military Medical Academy of Lodz,
Bieganski Hospital, Poland