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Book of Abstracts: Albany 2003

category image Albany 2003
Conversation 13
Abstract Book
June 17-21 2003

Model Describing the Formation of ?Bridges? Between Nuclear Acids in Liquid-crystalline Dispersions

This paper considers the family of the three experimental curves that describe the formation of ?bridges? between nuclear acids (NA) molecules fixed in the structure of liquid-crystalline dispersions (1, 2). In general three variants of ?bridge? formation are taken into consideration (Figure 1), all of them being based on the same chemical structure of the ?bridges?. We suppose their chelate polymer?s structure of interchanging daunomicin (DAU) molecules and copper ions. The differences is that the bridges begin and end by DAU molecules in the first variant, begin and end by copper ions bound to the base of NA in the second and have different kind of the ends in the third.


Figure 1: The three variants of the ?bridge? formation. The circles designate the bases of the NA , the rectangles designate the DAU molecules and the asterisks designate the copper ions.

A mathematical model describing the formation of the ?bridges? for each variant has been developed. For each variant we vary the value of interaction energy between DAU molecular and copper ion as well as the energy of DAU interaction with the NA, and also the length of the ?bridge?. We got the set of these parameters fitting best the experimental data. We demonstrate that every variant may describe the experimental curves with the same accuracy. The best fitting corresponds to the case of including more than two DAU molecules in the structure of the bridge.

Supported by RFBR grant #03-04-48212

V. F. Riabokon1,2
Yu. D. Nechipurenko1,4
S. V. Semenov3,4
Yu. M. Yevdokimov1,4

1V. A. Engelhardt Institute of Molecular Biology
Russian Academy of Sciences
Moscow, 119991 Russia
Phone: 7(095)135-9741
Fax: 7(095)135-1405
nech@imb.ac.ru
2Moscow Institute of Physics and Technology
State University
141700 Dolgoprudny
3RRC "Kurchatov Institute"
Moscow, 123182, Russia
4The Company ?Bioanalytical Technologies?
Moscow, 117049, Russia

References and Footnotes
  1. Yevdokimov Yu. M., Salyanov V. I., Zakharov M.A. Lab on a Chip 1, 35-41 (2001).
  2. Nechipurenko Yu. D., Salyanov V. I., Zakharov M.A., Yevdokimov Yu. M., Biophysics (Russian) 47, 600-606 (2002).