Book of Abstracts: Albany 2009
June 16-20 2009
© Adenine Press (2008)
Metabolome Analysis and Systems Biology
Institute for Advanced Biosciences of Keio University has recently developed a novel technology for high-throughput metabolome analysis. The technology is based on capillary electrophoresis time-of-flight mass spectrometry (CE/TOFMS) and it can simultaneously quantify a large amount of cellular metabolites ranged from 70 to 1,000 molecular weights . Metabolome analysis is applicable to various fields of biotechnology in the post-genomic era, such as medical diagnosis (blood, urine, tissue), food production (farm products, fermentation), and systems biology of model organisms (E.coli and other bacteria).
The metabolome technology has made ?multi-omics? analysis possible. We systematically obtained multi-omics data sets for Escherichia coli BW25113 and its single gene deletion mutants. Our data covers the metabolome (CE-TOFMS), proteome (western blot, shotgun proteomics, and 2D-DIGE), fluxome (GC-MS and NMR) and transcriptome (real time RT-PCR and DNA?microarray) .
In the area of medical diagnosis, we recently discovered a biomarker of acetaminophen-induced hepatotoxicity, ophthalmate being a sensitive?biomarker of glutathione depletion . In addition, Metabolome data have?been used to confirm simulation results of red blood cell metabolism .
References and Footnotes
Institute for Advanced Biosciences