Book of Abstracts: Albany 2003
June 17-21 2003
Melting of Complexes of DNA-cis-DDP in Acidic Media
Antitumor compound cis-diamminedichlorplatinum (II) (cis-DDP) is successfully applied in chemotherapy of tumors. There are enough works concerning to study of cis-DDP- DNA complex-formation in vivo. In many works is shown that in vivo cis-DDP is covalently linking with DNA in cell and inhibits its replication. On the other hand, it is also known, that at cell division there can be significant jumps of acidification to extremely expressed meanings of pH (9
The peculiarities of helix-coil transition of DNA at complex-formation with cis-diamminedichlorplatinum (II) (cis-DDP) have been investigated in acidic environment. It is shown, that observed features of behavior ΔT (pH) and Tm (pH) DNA at pH 2.8÷3.0 is possible to explain by formation in DNA of pseudo-ring structures at covalent linking of cis-DDP with DNA.
T. S. Haroutiunian1
1Department of Biophysics