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Albany 2015:Book of Abstracts

Albany 2015
Conversation 19
June 9-13 2015
©Adenine Press (2012)

Life at the Single Molecule Level: from Single Molecule Enzymology to Single Cell Genomics

DNA are single molecules carrying genetic codes in individual cells. For this reason gene expression is stochastic. Single-molecule gene expression experiments in live single cell, when combined with single molecule enzymology in vitro, allowed quantitative and mechanistic interpretations of the fundamental processes of gene expression. The fact that there are 46 different individual DNA molecules (chromosomes) in a human cell dictates that genomic variation occurs stochastically and cannot be synchronized among cells. To probe such genomic variations necessitates single-cell single-molecule measurements, which have been made possible recently, opening exciting opportunities to investigate and diagnose cancer, and to avoid genetic disorders in newborns.

Sunney Xie

Department of Chemistry and Chemical Biology
Harvard University
Cambridge MA
Peking University
Biodynamic Optical Imaging Center

xie@chemistry.harvard.edu