Book of Abstracts: Albany 2003
June 17-21 2003
Investigations of cis-Pt and its Analog DACH-Pt Interaction with DNA and Synthetic Oligonucleotides by Means of VCD, IR and CD Spectroscopy
We studied the interaction of cis-Pt and its more effective analog 1,2-diaminocyclo-hexane-platinum (II) (DACH-Pt) with natural calf thymus DNA as well as the interaction of DACH-Pt with the synthetic octamer duplex d(CCTGGTCC)·d(GGACCAGG) using Vibrational Circular Dichroism (VCD), IR and UV/CD. We measured these spectra at different temperatures between 25 to 70 °C for calf thymus DNA and between 4 to 55 °C for the octamer duplex. We obtained different spectral changes for DNA interacting with cis-Pt than with DACH-Pt. DACH-Pt showed strong destabilization of the octamer duplex. We were able to conclude that DACH-Pt possesses a higher ability for destabilizing the octamer duplex than ordinary cis-Pt. The results are compared for octamer duplex-DACH-Pt complex and octamer duplex-cis-Pt studied previously on our laboratory (1).
1Department of Chemistry