Book of Abstracts: Albany 2009
June 16-20 2009
© Adenine Press (2008)
Interrogating Single Nucleic Acid and Protein Molecules with a Nanopore
Advances in rational protein design and single-molecule technology allow for biochemical sampling at high temporal and spatial resolution and for the detection, manipulation, and exploration of individual molecules. We have developed a methodology for examining single biopolymer dynamics within a protein nanopore, a simple system that is highly pertinent to several more complex biological processes such as the translocation of DNA and proteins through transmembrane pores. The ionic current through a single protein nanopore was determined by single-channel electrical recordings in lipid bilayers. The results revealed the stochastic dynamics of biopolymers, such as their conformational fluctuations and interactions with other molecules, as well as the energetic requirements for their transition from one state to another. I will discuss various examples that demonstrate an accurate control of single proteins and protein pore-based nanostructures by using simple principles learned from physics and modern biology.