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Albany 2019: 20th Conversation - Abstracts

category image Albany 2019
Conversation 20
June 11-15 2019
Adenine Press (2019)

Interaction of Noscapine with Human Serum Albumin (HSA): A Spectroscopic and Molecular Modelling Approach

Noscapine, an opium derived anticancer agent manifests therapeutic applications and is compliant to beneficial modifications. In this paper, we study its interaction with human serum albumin (HSA) by different spectroscopic methods including UV-vis, fluorescence, circular dichroism and thermal denaturation and molecular docking. Binding and thermodynamics parameters support a feasible interaction of Nos-HCl with HSA. Further, type of interactions between Nos-HCl and HSA were also elucidated. The energy transfer between HSA and Nos-HCl was analyzed by Forster mechanism and lifetime measurements. Additionally, it was shown that Nos-HCl binds HSA in subdomain IIIA which constitute the drug binding Sudlow’s Site II. Preliminary results for binding site were deciphered via the site marker competitive experiments which were then confirmed using molecular docking analysis.

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References
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    M. Mahmoudian, P. Rahimi-Moghaddam, The anti-cancer activity of noscapine: a review., Recent Pat. Anticancer. Drug Discov. 4 (2009) 92–97.

    E. Pasquier, M. Kavallaris, Microtubules: A dynamic target in cancer therapy, IUBMB Life. 60 (2008) 165–170.

Heerak Chugh and
Ramesh Chandra

Department of Chemistry
University of Delhi
New Delhi 110007, India

Email: acbrdu@hotmail.com