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Mendel-Brno 2000

category image Volume: 17
Issue Number 6, Part 2
June 2000

Interaction of Anticancer Drug, Epirubicin with DNA

The interactions of some anticancer drugs with DNA have been studied by a variety of techniques [1-3] and in recent years, there is a growing interest in the electrochemical investigations of interactions between these drugs and DNA [4-7].

Epirubicin (EPR) which is an anticancer drug, is known to bind through intercalation, with the planar, aromatic group stacked between base pairs [7].

In this study, the interaction of EPR with double-stranded DNA (dsDNA) and single-stranded DNA (ssDNA) was studied electrochemically by using carbon paste electrode (CPE). The changes in the experimental parameters (buffer solution and pH, the concentration of EPR, the concentration of DNA and the accumulation time of EPR) was studied by using differantial pulse voltammetry; in addition, the detection limit and the reproducibility was determined. Both studies in different buffer solutions; acetate buffer (pH 4.8) and phosphate buffer (pH 7.4), it was observed for the interaction of EPR with dsDNA and ssDNA that the signal of bare electrode was higher than the signal of the dsDNA modified CPE. The observed signals caused by the interaction of EPR with DNA as the decreasing signals, respectively; CPE, ssDNA modified CPE, dsDNA modified CPE were found. The interaction of EPR with oligonucleotides was also studied for searching its use as a hybridization indicator. The similar results were also found with the oligonucleotides. It was determined that EPR can be used as a new promising hybridization indicator in optimum conditions.

References

[1] Lown, J.W., Hanstock, C.C., Bradley, R.D. and Scraba, D.G., Molecular Pharm., 25, (1984), 178-184.
[2] Fritzsche, H., Akhebat, A., Taillandier, E., Rippe, K. and Jovin, T.M., Nucleic Acids Research, 21(22), 1993, 5085-5091.
[3] Nunn, C.M., Meervelt, L.V., Zhang, S. et al., J. Mol. Biol., 222, (1991), 167-177.
[4] Fojta, M., Doffkova, R., Palecek, E., Electroanalysis, 8(5), (1996), 420-426.
[5] Wang, J., Ozsoz, M., Cai, X., Rivas, G., Shiraishi, H., Grant, D.H., Chicarro, M., Fernandes, J. R. and Palecek, E., Bioelectrochem. And Bioenerg., 45, (1998), 33 - 40.
[6] Perez, P., Teijeiro, C., Marin, D., Chemico-Biological Interactions, 117, (1999), 65-81.
[7] Xia, C., Guoli, S., Jianhui, J., Ruqin, Y., Anal. Lett., 32 (4), (1999), 717-727.

Arzum Erdem and Mehmet Ozsoz1

Faculty of Pharmacy, Department of Analytical Chemistry,
Ege University, 35100 Bornova-Izmir, Turkey
1e-mail: ozsozs@eczacilik.ege.edu.tr

$15.00