Homomeric Interaction of the Mouse Rad52 Protein
The Rad52 protein plays a crucial role in repairing DNA damage and homologous recombination, possibly by virtue of its ability to catalyze annealing of single-stranded DNA. In agreement with recent genetic data, we here present results based on the two-hybrid system, demonstrating that mouse Rad52p is able to form homomeric complexes. A small domain necessary and sufficient for the self-interaction is located in the conserved N-terminus of the protein. These data contribute to the important insights into the architecture of the multi-protein complex involved in recombinational DNA repair.
Lumir Krejci1,2 , Bo Thomsen1, Morten Dunq1, Ole Westergaard3, & Christian Bendixen1
1Department of Animal Breeding and Genetics,