Book of Abstracts: Albany 2007
June 19-23 2007
Formation of Pseudo-ring Structures in DNA Bound with cis-platinum
cis-diamminedichloroplatinum(II) (cis-DDP) form covalent adduct with DNA both in vivo and in vitro. In the present study, the UV-spectroscopic helix-coil transition method is used to analyze the changes of the high-molecular DNA double helix occurring due to the formation of complexes with cis-DDP at the concentrations of cis-DDP 0.00003 < rb < 0.003, where rb is the number of moles of bound DDP per mole of nucleotide.
The characteristic behavior of the high molecular DNA melting temperature Tm on concentration of cis-DDP shows that there exist three precisely expressed areas of concentration of ligand on efficiency of influence of cis-DDP on DNA:
Samvel G. Haroutiunyan1, *
1Departments of Molecular Physics