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Fast Mapping of Biomolecular Interfaces by Random Spin Labeling (RSL)

Random spin labeling (RSL) is a method for rapid mapping of biomolecular interaction surfaces using an interaction partner with SL and an interaction partner enriched in 13C or 15N nuclei for paramagnetic relaxation enhanced NMR-based detection. The SL reaction is conducted in a manner resulting in a heterogeneous reaction product consisting of different populations of the protein carrying a varying number of spin labels at different positions. Preparation of the paramagnetic probe is complete within a few hours and hence much faster than site selective SL. RSL is applicable to tightly interacting systems but shows its particular strength when applied to systems involving weak or transient contacts.

This article can be cited as:
M.J. Hartl, B.M. Burmann, S.J. Prasch, C. Schwarzinger, K. Schweimer, B.M., Wöhrl, P. Rösch, S. Schwarzinger. Fast Mapping of Biomolecular Interfaces by Random Spin Labeling (RSL)J. Biomol Struct Dyn 29(4), 793-798 (2012).

Maximilian J. Hartl1,3
Björn M. Burmann1
Stefan J. Prasch1
Clemens Schwarzinger2
Kristian Schweimer1,3
Birgitta M. Wöhrl1,3
Paul Rösch1,3
Stephan Schwarzinger1,3*

1Lehrstuhl Biopolymere, Universität Bayreuth, Universitätsstraße 30, 95440 Bayreuth, Germany
2Institut für Chemische Technologie Organischer Stoffe, Johannes Kepler Universität, Altenbergerstraße 69, 4040 Linz, Austria
3Research Center for Bio-Macromolecules, Universität Bayreuth, Universitätsstraße 30, 95440 Bayreuth, Germany

*Corresponding author:
Stephan Schwarzinger
Phone: 149 (0)921 552046
Fax: 149 (0)921 553684
stephan.schwarzinger@uni-bayreuth.de

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