Book of Abstracts: Albany 2005
Express-analysis of New Anti-tumor Compounds Binding with DNA
Almost all anti-tumor compounds, penetrating in a cell influence on structure of DNA, by which their anti-tumor effect is caused. Independently of mechanism of action (direct or undirected), the medicinal compounds influence on DNA changing it?s structure and properties.
The rather simple experimental method is offered: by investigating the structure, in particular spectrophotometrically determinating the thermodynamic parameters of binding (equilibrium constant and binding energy) as well as differential melting curves of modified DNA to make a correct choice from synthesized and biologically active anti-tumor medicinal compounds for the further investigation of mechanisms of their binding.
Antibiotics (mitoxantrone and ametontrone), alkylating anti-tumor compounds (phosphemide, cyclophosphane) applied in practice as anti-tumor preparations as well as testing compounds have been investigated by this way.
Yuri S. Babayan1,*