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Albany 2019: 20th Conversation - Abstracts

category image Albany 2019
Conversation 20
June 11-15 2019
Adenine Press (2019)

Evaluation and validation of synergistic effect of predicted Amyloid-Beta (Aβ) inhibitor by deep neural network.

Amyloid-beta 42 (Aβ-42) protein has been established in numerous investigations as high-profile risk factor associated with onset and progression of Alzheimer’s disease (AD). Extracellular senile plaques accumulation, synaptic degeneration and intracellular neurofibrillary tangles (NFT) were recorded as essential features that facilitate onset of Aβ-42 and results into AD. Hence, this study attempted a new screening technique to discover potential inhibitors against Aβ-42 by employing in silico deep neural network approach. In this method, Pubchem compounds library was screened and concluded wgx-50 as potential inhibitor of Aβ-42. Also, synergistic effect of wgx-50+gold nanoparticles (AuNP) induced significant inhibition of Aβ-42 against wgx-50 as control. Further, analysis of molecular docking, system biology approach and time course simulation revealed synergistic effect of wgx-50-AuNP complex as potential treatment for AD. Additionally, we purposed the biological circuit for the AD induced by Aβ-42 that can be employed to monitor the effect of drugs on AD.

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This work is supported by the Key Research Area Grant 2016YFA0501703 from the Ministry of Science and Technology of China, State Key Lab on Microbial Metabolism, and Joint Research Funds for Medical and Engineering from Scientific Research at Shanghai Jiao Tong University.

References
    A.C. Kaushik, et al. (2018). Deciphering the Biochemical Pathway and Pharmacokinetic Study of Amyloid βeta-42 with Superparamagnetic Iron Oxide Nanoparticles (SPIONs) Using Systems Biology Approach. Molecular neurobiology 55.4: 3224-3236.

    B.M. Austen, E.R. Frears & Davies, H. (2000). The use of Seldi ProteinChip™ Arrays to monitor production of Alzheimer's β‐amyloid in transfected cells. Journal of Peptide Science 6(9), 459-469.

Aman Chandra Kaushik
Dong Quing Wei

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Aman got his Ph D in 2016 from Gautam Buddha University under supervision of Dr. Shakti Sahi. Currently he is a post-doctoral fellow under supervision of Prof. Dong-Qing Wei, Shanghai Jiao Tong University, China.

State Key Laboratory of Microbial Metabolism
School of life Sciences and Biotechnology,
Shanghai Jiao Tong University
Shanghai 200240, China

Ph: (+86) 15618987739
Email: amanbioinfo@sjtu.edu.cn