Book of Abstracts: Albany 2011
June 14-18 2011
©Adenine Press (2010)
Effect of Millimeter Electromagnetic Waves on the Water-Saline Solutions of DNA and DNA-Ligand Complexes
There have been recently studies of various factors that affect the stability of DNA, DNA-ligand and RNA-ligand complexes (1-6). In this paper we examine the effect of radiation and ionic strength on the thermostability of DNA and DNA-ligand complexes. The effect of non-thermal (∼ 50μW/cm2) (50,3 and 64,5 GHz are resonant of water hexagonal structure frequencies) millimeter electromagnetic waves (MM EMW) on water-saline solutions of DNA and DNA-ligand complexes with EtBr and Hoechst 33258 is studied. Experimental data showed that radiation increased the thermostability of the investigated samples. The differences between the melting temperatures of radiated (Tmr) and non-radiated (Tmnr) DNA and its complexes with ligands (δ Tm= Tmr- Tmnr) depended on the ionic strength; the greater the ionic strength, the smaller is the δTm.
It is known, that the hydration of DNA decreases with increase of the Na+ concentration. Therefore, decrease of the value of δTm at high concentration of Na+ points to the non significant effect of the radiation of the structure of hydration shell of DNA and its complexes with the ligands, at these conditions. This conclusion is supported by our experimental results according to which the radiation of DNA and DNA-ligand complexes by non-resonant of water hexagonal structure frequency MMEMW (48,3 GHz) caused no changes in the thermostability of the samples at all investigated concentrations of Na+.
Experimental results obtained made it possible to suggest that radiation, like ionic strength of solutions, increases the thermostability of DNA and DNA-ligand complexes.
1Department of Biophysics of Yerevan State University
A.Manoogian str. 1, Yerevan 0025, Armenia