Book of Abstracts: Albany 2003
June 17-21 2003
Conformational Study of Virus Nucleic Acids: A Seira and Correlation Analysis Data
In the most cases the mechanisms of virus interaction with cell is known, however the formation of stable form cell after virus infection is not always understandable. MS-2 virus model study showed that RNA-virus interaction with cell-host could cause to mutation and formation of new virus forms.
IR spectroscopy is a powerful tool for characterization of DNA duplex in A, B, or Z-forms and its triplex organization. The DNA conformation has been analyzed with SEIRA (Surface enhanced infra red absorption) spectroscopy (1) and correlation analysis. FTIR spectra of mutated DNA-bacteriophages induced by MS-2 virus incorporated in E. coli have been registered. The intensity and frequency of phosphate bands (1000-1300 cm-1) and high frequency shoulder of the bases (1600-1700 cm-1) have been selected for matrix construction in principal component (PC) analysis.
The spectra of DNA phage _47 (phage numbers have been assigned by T. Pererva) have showed the appearance of RNA fraction. The Z-form has been found in the base vibration region at 1694 cm-1 and in the sugar region at 929 cm-1. Both bands are the marker of Z-form. A supposition about DNA triplex fragments in _23-2 phage has been proposed also. This fact could be proved by appearance the sugar vibrations at 986, 860, 835 cm-1. We have registered other mutating virus forms without Z-fragments and RNA contamination.
An attempt of nucleic acid conformation analysis algorithmization has been done. PC analysis showed that DNA from these viruses had fragments of different forms of double stranded DNA, single RNA and triplex.
1Institute of Physics of National Academy of Science of Ukraine