Albany 2013: Book of Abstracts
June 11-15 2013
©Adenine Press (2012)
Circular dichroism (CD) studies of the interaction between G-quadruplexes/ I-motif with saffron secondary active metabolites
Telomeric DNA contains some unique secondary structures, such as G-quadruplex and I-motif. These structures may be stabilized or changed by binding to specific proteins or small molecules. In continue with our previous studies on the interaction between crocin and crocetin, as the natural C20 carotenoids, and picrocrocin and safranal, as the natural monoterpene aldehydes, obtained from saffron and DNA (Bathaie et al. 2007; Hoshyar et al. 2008), herein, we report the in vitro effect of these saffron metabolites on the mentioned structures (Hoshyar et al. 2012). Circular dichroism (CD) data indicate crocetin has higher affinity for these structures. Safranal and crocin induce little change in the I-motif and G-quadruplex, respectively. The molecular docking confirms the experimental data and indicates the minor groove binding of ligands with G-quadruplex. Effect of these ligands on the stability of these structures are studying using some other techniques and determination of thermodynamic parameters.
R. Hoshyar, S.Z. Bathaie, M. Ashrafi (2008). DNA and Cell Biology, 27 (12), 1-9.
R. Hoshyar, S.Z. Bathaie, A. Kyani and M.F. Mousavi (2012). Nucleoside, Nucleotide, Nucleic Acids, 31 (11), 801-812.
Department of Clinical Biochemistry