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Albany 2019: 20th Conversation - Abstracts

category image Albany 2019
Conversation 20
June 11-15 2019
Adenine Press (2019)

Using DNA Origami to Decipher Spatial Effects in Biology

It is widely accepted that the biophysical context of ligands and receptors has significant impact of downstream signaling, however the concept is poorly understood due to difficulties in controlling and analyzing the microenvironment on the nanoscale. I will talk about how we think that DNA-nanotechnology can be of great help in both learning the tactile alphabet of cells by stimulation using protein decorated DNA-origami ‘nano-calipers’ and of help in understanding the binding of antibodies. I will also briefly present our recent work on ‘3D-printing’ DNA origami wireframe structures, a method that provides a way to make origami more accessible to experiments in physiological salt conditions.

References

    Binding to nanopatterned antigens is dominated by the spatial tolerance of antibodies, Shaw A, Hoffecker IT, Smyrlaki I, Rosa J, Grevys A, Bratlie D, Sandlie I, Michaelsen TE, Andersen JT and Högberg B, Nature Nanotechnology, 14, p. 184–190 (2019)

    DNA rendering of polyhedral meshes at the nanoscale, Benson E, Mohammed A, Gardell J, Masich S, Czeizler E, Orponen P and Högberg B, Nature, 523, p. 441–444 (2015)

    Spatial control of membrane receptor function using ligand nanocalipers, Shaw A, Lundin A, Petrova E, Fördős F, Benson E, Al-Amin A, Herland A, Blokzijl A, Högberg B and Teixeira A Nature Methods, 11, p. 841-846 (2014).

Björn Högberg

Department of Medical Biochemistry and Biophysics
Karolinska Institute
Stockholm, 171 77 Sweden

Ph: +46 8 524 870 36
Email: bjorn.hogberg@ki.se